Elimination of hop latent viroid upon developmental activation of pollen nucleases
Identifieur interne : 000802 ( Istex/Checkpoint ); précédent : 000801; suivant : 000803Elimination of hop latent viroid upon developmental activation of pollen nucleases
Auteurs : Jaroslav Matoušek [République tchèque] ; Lidmila Orctová [République tchèque] ; Josef Škopek [République tchèque] ; Karel Pešina [République tchèque] ; Gerhard Steger [Allemagne]Source :
- Biological Chemistry [ 1431-6730 ] ; 2008.
English descriptors
- Teeft :
- Acetate buffer, Agarose, Amino, Amino acids, Anther, Anther nucleases, Arabidopsis thaliana, Bicellular, Bicellular pollen, Bifunctional, Bifunctional nuclease, Biol, Bromide, Cdna, Cdna library, Cell biol, Ceske budejovice, Chem, Czech republic, Dark spots, Degradation, Developmental activation, Developmental changes, Dramatic decrease, Electrophoresed, Endonuclease, Ethidium, Ethidium bromide, Floral buds, Food chem, Generative, Generative cells, Germinating, Germinating pollen, Germination, Hbn1, Hbn1 mrna, Hlvd, Hlvd degradation, Hlvd detection, Hlvd elimination, Honys, Humulus, Humulus lupulus, Hybridization, Hybridized, Immature pollen, Intracellular, Itaya, Latent viroid, Light microscopy, Lupulus, Male gametophyte, Mass culture, Matousek, Maturating, Maturating pollen, Maturation, Mature pollen, Methods section, Microarray, Microarray data, Microspore, Molecular mass, Mrna, Nuclease, Nuclease activity, Nucleic acids, Nucleolytic, Nucleolytic activities, Patzak, Pollen, Pollen development, Pollen germination, Pollen maturation, Pollen mother cells, Pollen nuclease, Pollen nucleases, Pollen tissues, Pollen tube growth, Potato spindle tuber viroid, Present study, Previous work, Primer, Proof elimination, Pstvd, Radial diffusion method, Recombinant, Recombinant hbn1, Recombinant pollen nuclease, Rna, Rnase, Rnase activities, Secondary structure, Singh, Small rnas, Specific rnases, Ssdna, Sucrose, Thaliana, Thaliana pollen, Tobacco anthers, Total rnase activity, Tupy, Twell, Uninucleate, Uninucleate microspores, Viroid, Viroid degradation, Viroid elimination, Viroid propagation, Virol, Young bicellular pollen, Young pollen.
Abstract
Hop latent viroid (HLVd) is not transmissible through hop generative tissues and seeds. Here we describe the process of HLVd elimination during development of hop pollen. HLVd propagates in uninucleate hop pollen, but is eliminated at stages following first pollen mitosis during pollen vacuolization and maturation. Only traces of HLVd were detected by RT-PCR in mature pollen after anthesis and no viroid was detectable in in vitro germinating pollen, suggesting complete degradation of circular and linear HLVd forms. The majority of the degraded HLVd RNA in immature pollen included discrete products in the range of 230–100 nucleotides and therefore did not correspond to siRNAs. HLVd eradication from pollen correlated with developmental expression of a pollen nuclease and specific RNAses. Activity of the pollen nuclease HBN1 was maximal during the vacuolization step and decreased in mature pollen. Total RNAse activity increased continuously up to the final steps of pollen maturation. HBN1 mRNA, which is abundant at the uninucleate microspore stage, encodes a protein of 300 amino acids (34.1 kDa, isoeletric point 5.1). Sequence comparisons revealed that HBN1 is a homolog of S1-like bifunctional plant endonucleases. The developmentally activated HBN1 and pollen ribonucleases could participate in the mechanism of HLVd recognition and degradation.
Url:
DOI: 10.1515/BC.2008.096
Affiliations:
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type="ISSN">1431-6730</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acetate buffer</term><term>Agarose</term><term>Amino</term><term>Amino acids</term><term>Anther</term><term>Anther nucleases</term><term>Arabidopsis thaliana</term><term>Bicellular</term><term>Bicellular pollen</term><term>Bifunctional</term><term>Bifunctional nuclease</term><term>Biol</term><term>Bromide</term><term>Cdna</term><term>Cdna library</term><term>Cell biol</term><term>Ceske budejovice</term><term>Chem</term><term>Czech republic</term><term>Dark spots</term><term>Degradation</term><term>Developmental activation</term><term>Developmental changes</term><term>Dramatic decrease</term><term>Electrophoresed</term><term>Endonuclease</term><term>Ethidium</term><term>Ethidium bromide</term><term>Floral buds</term><term>Food chem</term><term>Generative</term><term>Generative cells</term><term>Germinating</term><term>Germinating pollen</term><term>Germination</term><term>Hbn1</term><term>Hbn1 mrna</term><term>Hlvd</term><term>Hlvd degradation</term><term>Hlvd detection</term><term>Hlvd elimination</term><term>Honys</term><term>Humulus</term><term>Humulus lupulus</term><term>Hybridization</term><term>Hybridized</term><term>Immature pollen</term><term>Intracellular</term><term>Itaya</term><term>Latent viroid</term><term>Light microscopy</term><term>Lupulus</term><term>Male gametophyte</term><term>Mass culture</term><term>Matousek</term><term>Maturating</term><term>Maturating pollen</term><term>Maturation</term><term>Mature pollen</term><term>Methods section</term><term>Microarray</term><term>Microarray data</term><term>Microspore</term><term>Molecular mass</term><term>Mrna</term><term>Nuclease</term><term>Nuclease activity</term><term>Nucleic acids</term><term>Nucleolytic</term><term>Nucleolytic activities</term><term>Patzak</term><term>Pollen</term><term>Pollen development</term><term>Pollen germination</term><term>Pollen maturation</term><term>Pollen mother cells</term><term>Pollen nuclease</term><term>Pollen nucleases</term><term>Pollen tissues</term><term>Pollen tube growth</term><term>Potato spindle tuber viroid</term><term>Present study</term><term>Previous work</term><term>Primer</term><term>Proof elimination</term><term>Pstvd</term><term>Radial diffusion method</term><term>Recombinant</term><term>Recombinant hbn1</term><term>Recombinant pollen nuclease</term><term>Rna</term><term>Rnase</term><term>Rnase activities</term><term>Secondary structure</term><term>Singh</term><term>Small rnas</term><term>Specific rnases</term><term>Ssdna</term><term>Sucrose</term><term>Thaliana</term><term>Thaliana pollen</term><term>Tobacco anthers</term><term>Total rnase activity</term><term>Tupy</term><term>Twell</term><term>Uninucleate</term><term>Uninucleate microspores</term><term>Viroid</term><term>Viroid degradation</term><term>Viroid elimination</term><term>Viroid propagation</term><term>Virol</term><term>Young bicellular pollen</term><term>Young pollen</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hop latent viroid (HLVd) is not transmissible through hop generative tissues and seeds. Here we describe the process of HLVd elimination during development of hop pollen. HLVd propagates in uninucleate hop pollen, but is eliminated at stages following first pollen mitosis during pollen vacuolization and maturation. Only traces of HLVd were detected by RT-PCR in mature pollen after anthesis and no viroid was detectable in in vitro germinating pollen, suggesting complete degradation of circular and linear HLVd forms. The majority of the degraded HLVd RNA in immature pollen included discrete products in the range of 230–100 nucleotides and therefore did not correspond to siRNAs. HLVd eradication from pollen correlated with developmental expression of a pollen nuclease and specific RNAses. Activity of the pollen nuclease HBN1 was maximal during the vacuolization step and decreased in mature pollen. Total RNAse activity increased continuously up to the final steps of pollen maturation. HBN1 mRNA, which is abundant at the uninucleate microspore stage, encodes a protein of 300 amino acids (34.1 kDa, isoeletric point 5.1). Sequence comparisons revealed that HBN1 is a homolog of S1-like bifunctional plant endonucleases. The developmentally activated HBN1 and pollen ribonucleases could participate in the mechanism of HLVd recognition and degradation.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>République tchèque</li></country><region><li>District de Düsseldorf</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Düsseldorf</li></settlement></list><tree><country name="République tchèque"><noRegion><name sortKey="Matousek, Jaroslav" sort="Matousek, Jaroslav" uniqKey="Matousek J" first="Jaroslav" last="Matoušek">Jaroslav Matoušek</name></noRegion><name sortKey="Orctova, Lidmila" sort="Orctova, Lidmila" uniqKey="Orctova L" first="Lidmila" last="Orctová">Lidmila Orctová</name><name sortKey="Pesina, Karel" sort="Pesina, Karel" uniqKey="Pesina K" first="Karel" last="Pešina">Karel Pešina</name><name sortKey="Skopek, Josef" sort="Skopek, Josef" uniqKey="Skopek J" first="Josef" last="Škopek">Josef Škopek</name></country><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Steger, Gerhard" sort="Steger, Gerhard" uniqKey="Steger G" first="Gerhard" last="Steger">Gerhard Steger</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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